李 毅，男，加拿大籍华裔，博士学位，教授，博士生导师，湖北省级人才，“黄鹤英才奖”获得者。1986年7月至1989年8月在华中师范大学生物系担任讲师。1998年被聘为加拿大魁北克国家科研中心Armand-Frappier研究所研究员，2003至2005年担任法国Bio-Espace Inc公司资深研究员和研究开发部主任，2006年回国担任华中师范大学生命科学学院教授，博士生导师及加拿大魁北克国家科研中心Armand-Frappier研究所讲座教授。主要学术兼职有国际病毒分类委员会细小病毒分会委员，PLoSONE编委，湖北省细胞生物学会理事，湖北省免疫学会常务理事，湖北省生物工程学会理事。2012年5月来武汉生物工学院工作，曾任生物工程系系主任、生物科学与技术系系主任、生命科学与技术学院院长、副校长。现任生命科学与技术学院名誉院长。
【联系方式】
办公电话：027-89648163
【教育经历】
1978年10月至1982年8月在华中师范大学生物学专业学习，获理学学士学位
1982年9月至1986年6月在华中师范大学分子病毒学专业学习，获理学硕士学位
1989年9月至1993年6月在法国蒙彼利埃II大学微生物学专业攻读博士，获理学博士学位
1995年至1998年在美国佛罗里达大学从事博士后研究
【研究方向】
细小病毒
【主持项目】
微小病毒B19磷脂酶在病毒感染中作用机制的研究 国家自然科学基金
兔出血热病毒RHDV重组疫苗的研究 国际合作
民办高校生物工程专业应用型人才培养模式改革 湖北省教育厅基金
人博卡病毒(HBoV1)非结构蛋白NP1在病毒复制过程中的作用及其机制 国家自然科学
抗人类细小病毒候选药物的研制 第三批黄鹤英才计划
抗人类细小病毒候选药物的筛选 武汉市创新人才开发资金
【代表性论文】
SELECTED PUBLICATIONS (*通讯作者)
1. Deng X, Li Y, Qiu J. 2014. Human bocavirus 1 infects commercially available primary human airway epithelium cultures productively. J Virol Methods. 195:112-9.
2. Li J, Yang Y, Dong Y, Li Y, Huang Y, Yi Q, Liu K, Li Y*. 2013. Key elements of the human bocavirus type 1 (HBoV1) promoter and its trans-activation by NS1 protein. Virol J. 10(1): 315.
3. Sun Bin, Cai Yingyue, Li Yongshu, Li Jingjing, Liu Kaiyu, Li Yi*, yang yongbo*. 2013. The Nonstructural Protein NP1 of Human Bocavirus 1 Induces Cell Cycle Arrest and Apoptosis in Hela Cells. Virology. 440(1): 75-83.
4. Deng Xuefeng, Dong Yanming, Yi Qianhui, Huang Yu, Zhao Dan, Yang Yongbo, Tijssen Peter, Qiu Jianming, Liu Kaiyu and Li Yi* . 2013. The determinants for the enzyme activity of human parvovirus B19 phospholipase A2 (PLA2) and its influence on cultured cells. PLOS ONE. 8(4): e61440.
5. Wang Yuan, Abd-Alla Adly M. M., Bossin Hervé, Li Yi*, Bergoin Max. 2013. Analysis of the transcription strategy of the Junonia coenia densovirus (JcDNV) genome. Virus Research. 174 (2) 101– 107.
6. Deng X, Yan Z, Luo Y, Xu J, Cheng F, Li Y*, Engelhardt JF, Qiu J. 2013. In vitro modeling of human bocavirus 1 infection of polarized primary human airway epithelia. J Virol. 87(7): 4097-102.
7. Luo Y, Deng X, Cheng F, Li Y, Qiu J. SMC1-mediated intra-S phase arrest facilitates Bocavirus DNA replication. J Virol. 87(7):4017-32. 2013.
8. Xu P, Cheng P, Liu Z, Li Y, Murphy RW, Wu K. 2012. Complete genome sequence of a monosense densovirus infecting the cotton bollworm, Helicoverpa armigera. J Virol. 86(19):10909. doi: 10.1128/JVI.01912-12.
9. Yanming Dong, Yu Huang, Ying Wang, Peng Xu, Yongbo Yang, Kaiyu Liu, Peter Tijssen, Jianxin Peng, Yi Li*. 2013. The effects of the 11 kDa protein and the putative X protein on the p6 promoter activity of Parvovirus B19 in Hela cells. Virus Genes. 46(1):167-9.
10. Qinfeng Huang, Xuefeng Deng, Ziying Yan, Fang Cheng, Yong Luo, Weiran Shen,Diana C. M. Lei-Butters, Aaron Yun Chen, Yi Li, Liang Tang, Maria So derlund-Venermo,John F. Engelhardt, Jianming Qiu. 2012. Establishment of a Reverse Genetics System for StudyingHuman Bocavirus in Human Airway Epithelia. PLoS Pathog. 8(8):e1002899.
11. Wei Wei, Wenxian Wu, Hui Ai, Zhongchao Gai, Yongbo Yang, Jianxin Peng, Huazhu Hong, Yi Li*, Kaiyu Liu. 2012. Baculovirus infection triggers apoptosis following amino acid starvation-induced autophagy in a permissive cell line SL-HP derived from Spodoptera litura. PLoS One. 2012;7(5):e37457.
12. Zhongchao Gai, Xiaojuan Zhang, Xia Wang, Jianxin Peng, Yi Li, Kaiyu Liu, Huazhu Hong. 2012. Differential proteomic analysis of Trichoplusia ni cells after continuous selection with activated Cry1Ac toxin. Cytotechnology, DOI 10.1007/s10616-012-9496-4.
13. Yasha Li, Yanming Dong, Jun Jiang, Yongbo Yang, Kaiyu Liu, Yi Li*. 2012. High prevelance of human parvovirus infection in patients with malignant tumors. Oncology Letters, 2012 3(3):635-640.
14. Jingjing Li, Bin Sun, Hu Han, Jinfeng Ouyang, Hanchao Yao, Yongbo Yang, Kaiyu Liu, Yi Li*. 2012. Application of baculovirus as a delivery vehicle for study of transcription and translation mechanism of parvovirus in non-permissive mammalian cells. Journal of Virological Methods. 183(1):1-7.
15. Qinfeng Huang, Ph.D.; Xuefeng Deng, M.S.; Sonja M Best, Ph.D.; Marshall E Bloom,Ph.D.; Yi Li, Ph.D. Jianming Qiu.2012. Internal Polyadenylation of Parvoviral Precursor mRNA Limits Progeny Virus Production，Virology. 426(2):167-77..
16. Wenxian Wu, Wei Wei, Maryem Ablimit, Yuan Ma, Tao Fu, Kaiyu Liu, Jianxin Peng, Yi Li, Huazhu Hong. 2011. Responses of two insect cell lines to starvation: Autophagy prevents them from undergoing apoptosis and necrosis, respectively. Journal of Insect Physiology. 57: (6,): 723-734.
17. Liu K, Li Y, Jousset FX, Zadori Z, Szelei J, Yu Q, Pham HT, Lépine F, Bergoin M, Tijssen P. 2011. The AdDNV densovirus of the European house cricket, Acheta domesticus, evolved a unique expression strategy among parvoviruses. J Virol. 85(19):10069-78.
18. Luo Y, Lou S, Deng X, Liu Z, Li Y, Kleiboeker S, Qiu J. 2011. Parvovirus B19 Infection of Human Primary Erythroid Progenitor Cells Triggers ATR-Chk1 Signaling, Which Promotes B19 Virus Replication. J. Virol. 85 (16): 8646-55.
19. Szelei J, Woodring J, Goettel MS, Duke G, Jousset FX, Liu KY, Zadori Z, Li Y, Styer E, Boucias DG, Kleespies RG, Bergoin M, Tijssen P. 2011. Susceptibility of North-American and European crickets to Acheta domesticus densovirus (AdDNV) and associated epizootics. J Invertebr Pathol. 106 (3):394-9.
20. Kaufmann B, El-Far M, Plevka P, Bowman VD, Li Y, Tijssen P, Rossmann MG. 2011. Structure of Bombyx mori Densovirus 1, a Silkworm Pathogen. J Virol. 85 (10):4691-4697.
21. Kaufmann B, Bowman VD, Li Y, Szelei J, Waddell PJ, Tijssen P, Rossmann MG. 2010. Structure of Penaeus stylirostris densovirus, a shrimp pathogen. J. Virol. 84(21):11289-96.
22. Szelei J, Liu KY, Li Y, Fernandes S, Tijssen P. 2010. Parvovirus 4-like virus in plasma products. Emerging Infectious Diseases. 16(3):561-4.
23. Deng, Xuefeng, Dong, Yanming, Zhu, yunyun, Zhao dan, Jian xinyan, Feng, Zhihong., Gai, zhongchao, Liu, Kaiyu，Li, Yi*. Evidence of the human parvovirus B19 circulating in childbearing-age women in Wuhan of Hubei province. 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011. May 10, 2011 - May 12, 2011. Wuhan, China.
24. Li, Jingjing, Wang, Yuan, Sun, Bin, Ouyang, Jinfeng, Han, Hu, Huang, Qinqin, Yang, Yongbo, Li, Yi*. Genome cloning of human bocavirus (HBoV1) and prospective study of NP1 gene transcription. 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011. May 10, 2011 - May 12, 2011, Wuhan,China.
25. Li, Yasha, Dong, Yanming, Xu, Guopeng, Xu, Peng, Zhao, Dan, Yue, Pengying, Wang, Ying, Liu, Kaiyu, Li, Yi*. The high risk of human parvovirus B19 infection in patients with malignant tumors. 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011. May 10, 2011 - May 12, 2011, Wuhan, China.
26. Ouyang, Jinfeng, Xiao, Jie, Han, Hu, Li, Jingjing, Sun, Bin, Ding, Sheng, Yang, Yongbo, Li, Yi*. Expression and antibody production of the NP1 and VP1u gene from Human Bocavirus. 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011. May 10, 2011 - May 12, 2011, Wuhan, China
27. Sun, Bin, Yue, Pengying, Li, Jingjing, Han, Hu, Ouyang, Jinfeng, Cai, Yingyue, Yang, Yongbo, Li, Yi*. Expression and purification of non-structural protein NS1 of human bocavirus and generation of its antibody. 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011. May 10, 2011 - May 12, 2011. Wuhan, China.
28. Guo, Zhen, Wang, Yuan, Yu, Qian, Xiao, Jie, Liu, Kaiyu, Wang, Ying, Commeyras, Auguste, Li, Yi*. Construction of recombinant rabbit hemorrhagichemorrhagic disease virus (RHDV) vaccine using myxoma virus (MV) as a Vector. 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011. May 10, 2011 - May 12, 2011. Wuhan, China.
29. Shigang Shan, Kaiyu Liu, Jianxin Peng, Hanchao Yao; Yi Li, Huazhu Hong.2009. Mitochondria are involved in apoptosis induced by ultraviolet radiation in lepidopteran Spodoptera litura cell line. Insect Science. 16 (6): 485-491.
30．Xuping Zhang, Wenjie Lan, Yujie Deng, Yuan Ma, Kaiyu Liu, Jianxin Peng, Yi Li and Huazhu Hong. 2008. Highly passage of spodoptera litura cell line causes its permissveness to baculovirus infection. Cytotechnology, 57: 233-243.
31. Lijun Liu, Jianxin Peng, Kaiyu Liu, Hong Yang, Yi Li, and Huazhu Hong. 2007. Influence of cytochrome c on apoptosis induced by Anagrapha (Syngrapha) falcifera multiple nuclear polyhedrosis virus (AfMNPV) in insect Spodoptera litura cells. Cell Biology International. 31: 996-1001.
32. Kaiyu Liu Qinghua Tang, Chong Fu, Jianxin Peng, Hong Yang, Yi Li and Huazhu Hong. 2007. Influence of Glucose Starvation on the Pathway of Death in Insect Cell Line Sl: Apoptosis Follows Autophagy. Cytotechnology. 54: 97-105.
33. Kouassi, N., Peng, J.x., Li,Y., Cavallaro,C., Veyrunes, J.C., and Bergoin, M. 2007. Pathogenicity of Diatraea saccharalis densovirus (DsDNV) to host insets and characterization of its viral genome. Virologica Sinica. 22 (1):53-61.
34. G. Fediere, M. El-Far, Y. Li, M. Bergoin, and P. Tijssen. 2004. Expression strategy of densonucleosis virus from Mythimna loreyi. Virology, 320 (1):181-9.
35. EL-Far M., Li Y., Fediere G., Abol-Ela S., and Tijssen P. 2004. Lack of infection of vertebrate cells by the densovirus from the maize worm Mythimna loreyi (MlDNV). Virus Research, 99 (1): 17-24.
36.Abd-Alla A, Jousset F. X., Li Y., Fediere G., Cousserans F., and Bergoin M. 2003. NS protein of the Junonia coenia densovirus (JcDNV) is essential for viral DNA replication in LD652 cell line and spodoptera littoralis larvae. J Virol, 78, 790-797.
37.Tijssen P., Li Y., El-Far M., Szelei, Letarte M; and Zadori Z. 2003. Organization and Expression strategy of the ambisense genome of densonucleosis virus of Galleria mellonella. J Virol, 77, 10357-10365.9.
38.R. El-Mergawy, Y. Li, M. El-Sheikh, M. El-Sayed, S. Abol-Ela, M. Bergoin, P. Tijssen, and G. Fediere. 2003. Epidemiology and biodiversity of the densovirus MlDNV in the field populations of Spodoptera littoralis and other noctuid pests. Bull. Fac. Agric., Cairo Univ., 54: 269-282
39.G. Fédière, Y. Li, Z. Zadori, J. Szelei, and P. Tijssen. 2002. Genome organization of Casphalia extranea densovirus (CeDNV), a new Iteravirus. Virology, 292, (2): 299-308.
40.Y. Li, Z. Zadori, H. Bando, R. Dubuc, G. Fédière, J. Szelei and P. Tijssen. 2001. Genome organization of the densovirus from Bombyx mori (BmDNV-1) and enzyme activity of its capsid. J. of General Virology, 82, 2821-2825.
41.Zoltan Zadori, Jozsef Szelei, Marie-Claude Lacoste, Yi Li, Sebastiaen Gariepy, Philippe Raymond, Marc Allaire, Ivan R. Nabi and Peter Tijssen. 2001. A viral phospholipase A2 is required for parvovirus infectivity. Developmental Cell, 1: 291-302.
42.Y. Li, F. X. Jousset, M. Bergoin, P. Tijssen. 2000. The transcription of the Acheta domesticus densovirus (AdDNV) is unique among the densovirus subfamily The infectious disease review, 2, 144.
43.Y. Li, G. Fédière, H. Bando, P. Tijssen. 2000. Relationship and genome organization of the densoviruses from Casphalia extranea (CeDNV) and Bombyx mori (BmDNV).The infectious disease review, 2, 140
44.Y. Li, S. Yuan, and R.W. moyer. 1998. The non-permissive infection of insect (Gypsy moth) LD-652 cells by vaccinia virus. Virology, 248, 74-82.
45.Y. Li, R.L. Hall, S. Yuan, and R. W. Moyer. 1998. High-level expression of the Amsacta moorei entomopoxvirus spheroidin depends on sequences within the gene. J. Gen. Virol. 79, 613-622.
46. Y. Li, R.L. Hall, and R. W. Moyer. 1997. Transient, nonlethal expression of genes in vertebrate cells by recombinant entomopoxviruses. J. Virol. 71, 9557-9562.
47. R.L.Hall, Y.Li, J.Feller, and R.W.Moyer. 1996. The Amsacta moorei entomopoxvirus spheroidin gene is improperly transcribed in vertebrate poxviruses. Virology, 224, 427-436.
48. Y. Li, F. X.Jousset, C.Giraud, F.Rolling, J. M.Quiot, M.Bergoin. 1996. A titration procedure of the Junonia coenia densovirus and quantitation of transfection by its cloned genomic DNA in four lepidopteran cell lines. J. of Virological Methods, 57, 47-60.
49. 董衍明, 黄玉, 彭建新,李毅*. 人细小病毒B19（Human Parvovirus B19, HPV B19）B19病毒11kDa蛋白对Hela细胞内NF-κB信号通路的影响. 生物工程学报.2013(7) 965-973.
50. 孙彬，李永淑，董衍明，刘凯于，杨勇波*,李毅*.人博卡病毒 HBoV1非结构蛋白NP1对细胞转录因子调控分析.微生物学报.2013.(7)737-745.
51.黄玉，赵丹，李毅*. 2012. 人细小病毒B19-UP1u保守区外C端氨基酸对sPLA2活性的影响化学与生物工程.29(7):32-35.
52. 刘凯于，彭建新，洪华珠，李毅*.浓核病毒研究概述. 2011. 中国生物防治学报. 27(4): 547-552
53. 李京京,孙彬,欧阳锦凤,陈莹,韩虎,刘凯于,李毅*.人类博卡病毒HBoV1基因组克隆及启动子活性分析[J]. 2011. 生物工程学报,27(6):909-916.
54. 陈莹，张丹，李京京，李毅*.人类博卡病毒结构蛋白基因VP2的克隆与表达. 2010.化学与生物工程. 27(3):67-69.
55.董衍明，王也，张楠，徐鹏，李毅*. 人细小病毒B19 非结构基因ns1 和7.5KD 基因的克隆与表达.中国病源生物学杂志，2010:5（7）,481-484
56. 邓雪锋，朱云云，菅新艳，冯志鸿，董衍明，李毅*. 2010. 用巢式PCR检测育龄妇女人群人细小病毒B19感染的研究，医学分子生物学杂志. 7(2):151-154.
57. 孙彬，李京京，陈莹， 李毅*. 2010. 人类博卡病毒（HBoV）非结构蛋白NS1基因的克隆、原核表达及多克隆抗体的制备，现代生物医学进展.Vol 10 (6), 1013-1016.
58. 谢马莉，于倩，李京京，李毅*. 2009. 家蚕浓核病毒BmDNV-1（伊那株）结构蛋白基因VP4的克隆、表达及抗体制备，华中师范大学学报. 43 (1): 103-109.
【其他成果】
Book or Chapter(专著)
Tijssen P., Bando H., Li Y., Jousset F.X., Zadori Z., Fédière G., El-Far M., Szelei J., Bergoin M.. 2006. Evolution of Densoviruses In” Parvoviruses”, Edit by Kerr, J.R.et al. London: Hodder Arnold.
Tijssen, P., Agbandje-Mckenna, M., Almentral, J., Bergoin, M., Flegel, T.W., Hedman, K., Kleischmidt, J., Li, Y., Pintel, D.J. and Tattersall, P. 2011. “Virus Taxonomy”. Ninth report of the International Committee on Taxonomy of Viruses. King, Andrew M.Q., Adams, Michael J., Carstens Eric B., Lefkowitz Elliot J.(Eds.), Family Parvoviridae (p405-425). Elsevier.
PATENTS （专利）
1. Entomopoxvirus Expression System, United States, Patent No. 5,935,777, August 10,1999, Inventor : Moyer; Richard W., Hall; Richard L.,Gruidl; Michael E., and Li; Yi. Assignee: University of Florida, Gainesville, FL
2. Entomopoxvirus-Vertebrate Gene Delivery Vector and Method, United States, Patent No. 6,106,825. August 22, 2000.Inventor: R.W. Moyer, Y. Li, R.L. Hall.Assignee: University of Florida, Gainesville, FL
3. Materials and Methods for Delivery and Expression of Heterologous DNA in Vertebrate Cells. EPO Patent No. 1,079,864, October 3, 2003.Inventors: R. W. Moyer, Y. Li. Assignee: University of Florida, Gainesville, FL
4. Hybrid-ionone and curcumin molecules as anticancer
agents Patent Application in United States No. 13131275, May 26,
2011.Inventors: Jian Hui Wu, Gerald Batist, Jinming Zhou, Guoyan Geng, Rongtuan
Lin, Yi Li.
